内蒙古地区临床型奶牛子宫内膜炎葡萄球菌毒力基因检测及耐药性研究

doi:10.11843/j.issn.0366-6964.2014.02.019

Abstract

Abstract:

The aim of the study was to investigate the distribution of superantigen genes and the profile of antimicrobial resistance in staphylococcal strains isolated from dairy cows with clinical endomitritis in Inner Mongolia.A total of 260 purulent secretions collected from bovine with clinical endomitritis were used for the isolation of Staphylococci and subsequently，the suspected strains were molecularly identified to species level.The distribution of staphylococcal superantigen toxin genes (SEs and TSST-1) was investigated by PCR.The MICs of 16 antimicrobial agents and 3 pairs of antimicrobial agents combinations to the isolates were determined by broth micro-dilution method.PCR was conducted to detect the presence of genes conferring resistance to erythromycin and tetracycline.A total of 127 Staphylococcus isolates belonging to 10 varied species，including 53 SA (41.7%) and 74 CNS (58.3%)，were isolated from 260 clinical samples.Eighty isolates (63.0%) were found to harbor at least one staphylococcal superantigen toxin gene and the prevailing of 17 different combinations of superantigen toxins genes was observed.Sej (38.6%) was the most frequently found and the “sec+sej+sen”(33.3%) was the most commonly encountered combination.The highest resistance was observed in penicillin (79.5%) and ampicillin (71.7%).The resistance rate of the isolates to cephalosporins，macrolide and tetracycline，fluoroquinolones and 3 antibiotic combinations were approximately 60%，40% 20%，and 10% respectively.None of isolates were resistant to vancomycin.The resistance to erythromycin of the isolates was mainly contributed by the ermC (46.4%) and ermB (37.5%) gene and ermA gene was not found in any isolates.TetK (70.0%) and tetM (10.0%) gene conferred the resistance of the isolates to tetracycline.Twenty-two CNS isolates were phenotypic and molecularly identified as MRS.The resistance rate of MRS isolates to penicillin (P<0.01)，ampicillin (P<0.01)，clarithromycin (P<0.01)，erythromycin (P<0.01) and cefotaxime (P<0.05) were significantly higher than MSS.The results indicated that the prevalence of the superantigen genes in staphylococci isolated from bovine with clinical endometritis in Inner Mongolia was complicated and the antimicrobial resistance of the isolates was serious，meanwhile，the prevalence of MRCNS was observed in bovine originated staphylococcal isolates，suggesting that the resistance of the isolates to antimicrobial agents was one of the crucial reasons causing the failure of treatment for bovine clinical endomitritis in Inner Mongolia.

CLC Number:

S852.611

ZHAO Jun-li，LI Pei-feng，LI Zhi-fang，GUAN Hong，DING Yue-xia. Characterization of Virulent Genes and Antibiotic Resistance of Staphylococcus Isolates from Bovine with Clinical Endomitritis in Inner Mongolia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 295-304.

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References

［1］GAUTAM G，NAKAO T，YUSUF M，et al.Prevalence of endometritis during the postpartum period and its impact on subsequent reproductive performance in two Japanese dairy herds ［J］.Anim Reprod Sci，2009，116(34)：175-187.
［2］SHELDON I M，CRONIN J，GOETZE L，et al.Defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle ［J］.Biol Reprod，2009，81(6): 1025-1032.
［3］陆承平.兽医微生物学［M］.北京：中国农业出版社，2001.
［4］TAPONEN S，PYÖRÄLÄ S.Coagulasenegative staphylococci as cause of bovine mastitis-Not so different from Staphylococcus aureus? ［J］.Vet Microbiol，2009，134(1-2)：29-36.
［5］WANG S C，WU C M，XIA S C，et al.Distribution of superantigenic toxin genes in Staphylococcus aureus isolates from milk samples of bovine subclinical mastitis cases in two major diary production regions of China ［J］.Vet Microbiol，2009，137(3-4)：276-281.
［6］张静，于三科，王新，等.原料乳和临床乳房炎金黄色葡萄球菌毒力基因检测及药敏分析［J］.中国兽医学报，2012，32（5）：759-764，770.
［7］LOEFFLER A，BOAG A K，SUNG J，et al.Prevalence of methicillin-resistant Staphylococcus aureus among staff and pets in a small animal referral hospital in the UK ［J］.J Antimicrob Chemother，2005，56(4)： 692-697.
［8］ITO T，KATAYAMA K，ASADA K，et al.Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus ［J］.J Antimicrob Chemother，2001，45(5)：1323-1336.
［9］FESSLER A T，BILLERBECK C，KADLEC K，et al.Identification and characterization of methicillin-resistant coagulase-negative staphylococci from bovine mastitis［J］.J Antimicrob Chemother，2010，65(8)：1576-1582.
［10］HISATA K,KUWAHARAARAI K,YAMANOTO M，et al.Dissemination of methicillin-resistant staphylococci among healthy Japanese children ［J］.J Clin Microbiol，2005，43（7）：3364-3372.
［11］SHELDON I M，LEWIS G S，LEBLANC S，et al.Defining postpartum uterine disease in cattle ［J］.Theriogenology，2006，65(8)：1516-1530.
［12］SANTOS T M，CAIXETA L S，MACHADO V S，et al.Antimicrobial resistance and presence of virulence factor genes in Arcanobacterium pyogenes isolated from the uterus of postpartum dairy cows ［J］.Vet Microbiol，2010，145(1-2)：84-89.
［13］YUGUEROS J，TEMPRANO A，BERZAL B，et al.Glyceraldehyde-3-phosphate dehydrogenase-encoding gene as a useful taxonomic tool for Staphylococcus spp ［J］.J Clin Microbiol，2000，38（12）：4351-4355.
［14］LINA G，QUAGLIA A，REVERDY M E，et al.Distribution of genes encoding resistance to macrolides，lincosamides，and streptogramins among Staphylococci ［J］.Antimicrob Agents Chemother，1999，43（5）：1062-1066.
［15］LIM J A，KWON A R，KIM S K，et al.Prevalence of resistance to macrolie，lincosamide and streptogramin antibiotics in Gram-positive cocci isolated in a Korean hospital ［J］.J Antimicrob Chemother，2002，49(3)：489-495.
［16］JONES C H，TUCKMAN M，HOWE A Y，et al.Diagnostic PCR analysis of the occurrence of methicillin and tetracycline resistance genes among Staphylococcus aureus isolates from phase 3 clinical trials of tigecycline for complicated skin and skin structure infections ［J］.Antimicrob Agents Chemother，2006，50（2）：505-510.
［17］JOHNSON W M，TYLER S D，EWAN E P，et al.Detection of genes for enterotoxins，exfoliative toxins，and toxic shock syndrome toxin 1 in Staphylococcus aureus by the polymerase chain reaction ［J］.J Clin Microbiol，1991，29(3): 426-430.
［18］CHEN T R，CHIOU C S，TSEN H Y.Use of novel PCR primers specific to the genes of staphylococcal enterotoxin G，H，I for the survey of Staphylococcus aureus strains isolated from food-poisoning cases and food samples in Taiwan ［J］.Int J Food Microbiol，2004，92(2)： 189-197.
［19］OTE I，TAMINIAU B，DUPREZ J N，et al.Genotypic characterization by polymerase chain reaction of Staphylococcus aureus isolates associated with bovine mastitis ［J］.Vet Microbiol，2011，153(3-4)：285-292.
［20］Clinical and Laboratory Standards Institute (CLSI).Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard［S］.Ninth Edition.Clinical and Laboratory Standards Institute，Wayne，PA，CLSI document M07-A9，2012.
［21］Clinical and Laboratory Standards Institute (CLSI).Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Second Informational Supplement［S］.Clinical and Laboratory Standards Institute，Wayne，PA，CLSI document M100S22，2012.
［22］ZHANG K，MCCLURE J A，ELSAYED S，et al.Novel multiplex PCR assay for characterization and concomitant subtyping of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus ［J］.J Clin Microbiol，2005，43（10）：5026-5033.
［23］VON EIFF C，PETERS G，HEILMANN C.Pathogenesis of infections due to coagulase-negative staphylococci ［J］.Lancet Infect Dis，2002，2(11): 677-685.
［24］PYÖRÄLÄ S，TAPONEN S.Coagulase-negative staphylococci-Emerging mastitis pathogens ［J］.Vet Microbiol，2009，134(1-2)：3-8.
［25］夏胜超，吴聪明，王绍琛，等.奶牛乳房炎病例中金黄色葡萄球菌毒素基因的检测［J］.中国兽医杂志，2007，43（4）：23-25.
［26］KUROISHI T，KOMINE K，KAI K，et al.Concentrations and specific antibodies to staphylococcal enterotoxin-C and toxic shock syndrome toxin-1 in bovine mammary gland secretions，and inflammatory response to the intramammary inoculation of these toxins ［J］.J Vet Med Sci，2003，65(8):899-906.
［27］KONDO Y，ITO T，MA X X,et al.Combination of multiplex PCRs for staphylococcal cassette chromosome mec type assignment: rapid identification system for mec，ccr，and major differences in junkyard regions ［J］.Antimicrob Agents Chemother，2007，51（1）：264-274.
［28］TULINSKI P，FLUIT A C，WAGENAAR J A，et al.Methicillin-resistant coagulase-negative staphylococci on pig farms as a reservoir of heterogeneous staphylococcal cassette chromosome mec elements ［J］.Appl Environ Microbiol，2012，78(2)：299-304.

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Characterization of Virulent Genes and Antibiotic Resistance of Staphylococcus Isolates from Bovine with Clinical Endomitritis in Inner Mongolia

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